Why do we use HGH in a Liqiud format?

WHY DO WE USE HGH IN A LIQUID FORMAT?

These kinds of claims are frequently made, such as "The stability of growth hormone in a liquid condition is only achievable for many days and only when stored in a refrigerator." Let's assume straight now that growth hormone is a sophisticated, intricate, and delicate product, proving that this assertion was accurate. Before 2007, Let's examine why this won't be relevant in 2022.GH is more steady than previously believed, and there are plenty of scientifically valid studies to support this:

For instance, it was discovered in a study by an independent laboratory, Daewoong Co Ltd, that the harm a hormone sustains when it is in solution for an extended period of time is homogenous and specific:

Decomposition products, such as those that are deamidated or sulfoxylated, as well as polymeric or dimeric forms, can all form in solutions. Deamidation, which particularly takes place at the asparagine residue at position 149 by direct hydrolysis, is the most common chemical breakdown reaction of human growth hormone.It suggests that the most destructive chemical reactions were discovered rather than molecular degradation in accordance with the domino principle, as it was previously supposed. Thestability of the GH solution will increase to a new level if these processes are stopped or slowed down.

And this "trick" was pulled off by powerful pharmaceutical companies, who introduced liquid growth hormone formulations to the market and proved their efficacy through peer-reviewed research and patent approval technology today.Growth hormone was severely dehydrated before being converted to a dry form, and even after adapting the lyophilization procedure specifically for somatotropin (the medication is dried, frozen, and then placed in a vacuum chamber, where the solvent is sublimated), the GR molecule's "fragility" remained very noticeable. The requirement for routine solution preparation caused the end user the same irritation.

However, empirical research led specialists to the conclusion that if we alter the manufacturing process, lyophilization and sublimation are not required—it will simply remain in a stable liquid form. The liquid that growth hormone is discharged into throughout the synthesis process has a different chemical makeup. After stabilising agents were added, the resulting solution was sufficiently stable that all testing demonstrated that the liquid form was superior to the dry form in terms of the percentage of GH molecules that were destroyed as well as resilience to outside effects (including lyophilization and sublimation).

As a result, the most "harmful" steps in the drug's manufacturing process are skipped, and the finished liquid form is even more practical for the user. The remedy proved to be so successful that it passed every test and was released onto the market as the finished item. In order to produce growth hormone in a liquid form, all producers are currently switching to the new standard. Despite the fact that dry growth hormone and liquid growth hormone are the same substance, there are significant differences in the production process and the end product's characteristics that should be noted at this point. It's crucial to understand that liquid growth hormone is already manufactured in this manner, and that only minimally damaging forces are used to its delicate molecules during creation.

Disclosing patent trade secrets:

The earlier-mentioned study is only one among several. In essence, scientists had to make a medicine that was both efficient and safe for human use in a stable, usable form. As you can see, this growth hormone-containing liquid is a much more sophisticated alternative to the bacteriostatic water for injection available at your local pharmacy, which is needed to dissolve the antiquated dry form.

Although the reader is probably not interested in delving deeply into the specifics of such a small field, there is a wealth of information available to those who are: For instance, an aqueous formulation of human growth hormone including mannitol, a buffer, and a non-ionic surfactant is described in US Patent No. US 6,448,225 relating to Genentech's Nutropin AQ, with citrate buffer being specified as desirable. A stable aqueous formulation of human growth hormone is described in Korean Patent Application No. 10-1999-0001217. This formulation includes a buffer solution created using a buffer solution of low to moderate acidity, preferably a maleate buffer containing benzalkonium chloride.

The perpetual motion machine, for instance, is impossible to patent since the process requires substantiating the "effectiveness" of the innovation, not merely its "uniqueness" - another benefit of liquid growth hormone!Stabilizers function!Let's move a little bit aside. Growth hormone is a relatively complex molecule (it has 191 amino acids, each of which has 10 to 30 atoms), making it very challenging to examine its activity in solution using analytical or synthetic approaches. The unique strategy used in this field is an attempt to objectively address the question of why endogenous GR in the human body breaks down more slowly than anticipated and continues to function at 100%. The causes of growth hormone's greater blood stability than anticipated, even at body temperature (and we all know that GH doesn't enjoy high temperatures), are being investigated by scientists. It was discovered in the early 2000s that proteins dissolved in plasma "poached" the damaging dipole properties of the liquid and that blood pH has an impact on the stability of the GR molecule.What qualities do these possess? Also, this is an important thing.

The fact that a water molecule is a dipole—having a positive charge on one side and two negative charges on the other—allows it to "attach" to other molecules and, when subjected to continuous thermal Brownian motion, even destroy crystal lattices. It also exhibits solvent-like properties, though not the weakest.

The electrostatic contact between particles (ions, molecules) of a solute and a solvent is known as solvation (from the Latin solvo, "I dissolve"). Hydration is the process of solvating in aqueous fluids. Solvates are the molecular aggregations produced by solvation (in the case of water, hydrates). Therefore, solvation results in the fact that the type of solvent impacts the reaction mechanism, defines the relative stability of tautomers, conformers, and isomers, and can change the rate of chemical reactions up to 109 times (!).

In other words, we may manage the stability of the molecules in our major natural solvent by carefully choosing "additives". Since products that have performed well in vitro might not be suitable for use in humans, this is a very crucial and labor-intensive task!

These methods for dealing with HGH instability in solutions were first discussed in the early 2000s, and judging by the fact that even major pharmaceutical companies have patented, certified, and marketed liquid forms, it already suggests the existence of significant know-how in this field. Some patents have since expired, and it is now possible to investigate, comprehend, and improve the secret technologies of major pharmaceutical companies.

Scientific research demonstrates a brief overview as follows:

Growth hormone is therapeutically utilised to treat burn victims in China and the United States; it vastly speeds up patients' healing and recovery.The patients were split into two groups, one of which received HGH in dry form and the other in liquid form. In the group receiving liquid growth hormones, the average recovery time was 38 days (minimum 19, maximum 48), whereas in the group receiving dry growth hormones, the average recovery time was 30 days (minimum 15, maximum 60)! Scientists stress that there was no clinical difference between the liquid and dry form within the bounds of mathematical error, as well as the enormous difference in cases recorded before to the clinical use of GH, when recovery was much slower.The convenience of application and stability of the liquid form are definitely advantages over the dry version, which scientists have not shown to be more effective. Liquid form is useful. Proven.

Let's summarise:

In advance, no, liquid growth hormone is not a powder that has been "specially" dissolved. It is a preparation manufactured with a distinct technology that does not require drying before use or storage, minimising the risk of molecular damage.bAlthough the efficacy of liquid growth hormone is comparable to that of dry forms, the liquid form has a competitive advantage over the dry form on the market and is increasingly displacing it.